Search results for "3AB-OS cells"

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Mutant p53 gain of function can be at the root of dedifferentiation of human osteosarcoma MG63 cells into 3AB-OS cancer stem cells

2014

Osteosarcoma is a highly metastatic tumor affecting adolescents, for which there is no second-line chemotherapy. As suggested for most tumors, its capability to overgrow is probably driven by cancer stem cells (CSCs), and finding new targets to kill CSCs may be critical for improving patient survival. TP53 is the most frequently mutated tumor suppressor gene in cancers and mutant p53 protein (mutp53) can acquire gain of function (GOF) strongly contributing to malignancy. Studies thus far have not shown p53-GOF in osteosarcoma. Here, we investigated TP53 gene status/role in 3AB-OS cells-a highly aggressive CSC line previously selected from human osteosarcoma MG63 cells-to evaluate its involv…

HistologyTumor suppressor genePhysiologyEndocrinology Diabetes and MetabolismApoptosisIn situ hybridizationBiologyTNF-Related Apoptosis-Inducing LigandCell MovementCancer stem cellCell Line TumorSettore BIO/10 - BiochimicaBiomarkers TumormedicineHumansNeoplasm Invasiveness3AB-OS cells CSCs Cancer cell dedifferentiation Cancer stem cells FISH Fluorescent in situ hybridization GOF Gain of function Human osteosarcoma MMPs Matrix metalloproteinases Mutant p53 Mutant p53 gain of function Mutp53 OS OsteosarcomaClonogenic assayTumor Stem Cell AssayCell ProliferationMembrane Potential MitochondrialOsteosarcomaCancerReceptors Death DomainCell DedifferentiationCell cyclemedicine.diseaseMolecular biologyAmino Acid SubstitutionProto-Oncogene Proteins c-bcl-2Gene Knockdown TechniquesMutationNeoplastic Stem CellsCancer researchOsteosarcomaEctopic expressionTumor Suppressor Protein p53Bone
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MicroRNA-29b-1 impairs in vitro cell proliferation, self‑renewal and chemoresistance of human osteosarcoma 3AB-OS cancer stem cells

2014

Osteosarcoma (OS) is the most common type of bone cancer, with a peak incidence in the early childhood. Emerging evidence suggests that treatments targeting cancer stem cells (CSCs) within a tumor can halt cancer and improve patient survival. MicroRNAs (miRNAs) have been implicated in the maintenance of the CSC phenotype, thus, identification of CSC-related miRNAs would provide information for a better understanding of CSCs. Downregulation of miRNA-29 family members (miR-29a/b/c; miR‑29s) was observed in human OS, however, little is known about the functions of miR-29s in human OS CSCs. Previously, during the characterization of 3AB-OS cells, a CSC line selected from human OS MG63 cells, we…

cancer stem cellsHomeobox protein NANOGCancer Research3AB-OS cells; Cancer stem cells; MicroRNA; MicroRNA-29b-1; Multidrug resistance; Osteosarcoma; Bone Neoplasms; Cell Line Tumor; Cell Movement; Cell Proliferation; Drug Resistance Neoplasm; Gene Expression Regulation Neoplastic; Humans; MicroRNAs; Neoplasm Invasiveness; Osteosarcoma; Cancer Research; OncologyDrug ResistanceBone NeoplasmsBiologyCell LineSOX2multidrug resistanceCell MovementCancer stem cellCell Line TumorSettore BIO/10 - BiochimicamicroRNAmedicineHumansNeoplasm InvasivenessClonogenic assaymicroRNA-29b-1Cell ProliferationNeoplasticOsteosarcomaTumormicroRNAOncogeneCancer3AB-OS cellsArticlesCell cyclemedicine.diseaseGene Expression Regulation Neoplasticosteosarcoma cancer stem cells microRNA microRNA-29b-1 multidrug resistance 3AB-OS cellsMicroRNAsGene Expression RegulationOncologyDrug Resistance NeoplasmImmunologyCancer researchNeoplasm
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